Neomycin ELISA test Kit 1. Principle This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen precoated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density ( OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.5 ppb Detection limit Honey................................................................. ..................................... 10 ppb Chicken/ liver, pork/ liver, fish, shrimp....................................................... 10 ppb Recovery rate Chicken, pork, fish, shrimp....................................................................85 ± 15% Liver.................................................................. .....................................70± 10% Cross-reaction rate Neomycin............................................................... ................................... 100% Streptomycin........................................................... ................................ < 0.1% Gentamicin............................................................. ................................. < 0.1% Dihydrostreptomycin.................................................... ........................... < 0.1% Kanamycin.............................................................. ................................ < 0.1% Tobramycin............................................................. .................................< 0.1% Sisomicin.............................................................. ...................................< 0.1%