Swine Foot and Mouth Disease Distinguishing Test Kit 1. Usage Swine Foot and Mouth Disease Distinguishing ELISA Test kit is used for detection the nonstructural 3ABC-protein of swine foot and mouth disease ( FMD-3ABC) antibodies in the serum, and differentiation naturally infected from vaccined swine. 2. Brief Introduction The test kit is made of the antigen coated microplate, enzyme conjugation( HRP goat-anti-pig IgG) and other reagents, which applies the indirect ELISA principle to test the antibodies against FMD-3ABC in the serum. 3. Reagents 1 Antigen coated microplate..................................................... 2 pieces ( 96 wells) 2 Negative ( green lid) / positive ( red lid) serum................. 1 tube each ( 1 mL/ tube) 3 Anti-pig IgG-HRP combination( yellow lid) ........................ 1 bottle ( 22 mL/ bottle) 4 20× concentrated washing solution ( white lid) ................. 1 bottle ( 30 mL/ bottle) 5 Substrate A ( purple lid) / B ( yellow lid) ...................... 1 bottle each ( 12 mL/ bottle) 6 Stop solution ( blue lid) ......................................................1 bottle ( 12 mL/ bottle) 7 Sample diluent solution ( transparent lid) ..........................1 bottle ( 50 mL/ bottle)